Synthesis of acetylcholine receptors by cultured chick myotubes and denervated mouse extensor digitorum longus muscles.
نویسندگان
چکیده
Mono-[125I]iodo- alpha - bungarotoxin - receptor complexes extracted from chick myotube cultures as well as from adult denervated extensor digitorum longus muscles of the mouse have been banded at their buoyant density in gradients of metrizamide-deuterium oxide. When cultures or denervated adult muscles are preincubated in media containing 2H- or 13C-substituted amino acids under conditions of active receptor accumulation, the mono-[125I]iodo-alpha-bungarotoxin-receptor complexes have an increased buoyant density and band in a position of higher density in the gradient relative to a marker of mono-[131I]iodo-alpha-bungarotoxin-receptor complexes extracted from cells preincubated in normal media. It is concluded that the accumulation of receptors on the surfaces of cultured chick myotubes and on the non-synaptic surfaces of extensor digitorum longus muscles following denervation are the result of de novo synthesis.
منابع مشابه
Regulation of protein metabolism by a physiological concentration of insulin in mouse soleus and extensor digitorum longus muscles. Effects of starvation and scald injury.
1. Although high concentrations of insulin affect both synthesis and degradation of skeletal-muscle protein, it is not known to what extent these effects occur with physiological concentrations. The effects of a physiological concentration of insulin (100 mu units/ml) on muscle protein synthesis, measured with [3H]tyrosine, and on muscle protein degradation, measured by tyrosine release in the ...
متن کاملUltrastructure of the 2-months denervated rat leg muscle
Ultrastructure of slow (soleus, SOL) and fast (extensor digitorum longus, EDL) rat leg muscles were examined two months after denervation when progress of atrophy slow down, and it enters in some more stable stage. In the experimental muscles the mass decreased to about 25% of the control value. Muscle fibres were decreased in size considerably, but not uniformly. The most muscle fibres were pr...
متن کاملElectrical stimulation attenuates denervation and age-related atrophy in extensor digitorum longus muscles of old rats.
Skeletal muscles of old rats and elderly humans lose muscle mass and maximum force. Denervation is a major cause of age-related muscle atrophy and weakness, because denervated fibers do not contract, and undergo atrophy. At any age, surgical denervation causes even more dramatic muscle atrophy and loss in force than aging does. Electrical stimulation that generates tetanic contractions of dener...
متن کاملExcitation-contraction coupling in skeletal muscle of a mouse lacking the dihydropyridine receptor subunit gamma1.
1. In skeletal muscle, dihydropyridine (DHP) receptors control both Ca(2+) entry (L-type current) and internal Ca(2+) release in a voltage-dependent manner. Here we investigated the question of whether elimination of the skeletal muscle-specific DHP receptor subunit gamma1 affects excitation-contraction (E-C) coupling. We studied intracellular Ca(2+) release and force production in muscle prepa...
متن کاملMuscle RANK is a key regulator of Ca2+ storage, SERCA activity, and function of fast-twitch skeletal muscles.
Receptor-activator of nuclear factor-κB (RANK), its ligand RANKL, and the soluble decoy receptor osteoprotegerin are the key regulators of osteoclast differentiation and bone remodeling. Here we show that RANK is also expressed in fully differentiated myotubes and skeletal muscle. Muscle RANK deletion has inotropic effects in denervated, but not in sham, extensor digitorum longus (EDL) muscles ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 73 1 شماره
صفحات -
تاریخ انتشار 1976